Somkuti G A, Babel F J, Somkuti A C
Appl Microbiol. 1969 Jun;17(6):888-92. doi: 10.1128/am.17.6.888-892.1969.
Culture filtrates of Mucor pusillus NRRL 2543 contained hydrolytic enzymes that attacked native cellulose, acid-swollen cellulose, carboxymethylcellulose, and cellobiose. The distribution profiles of cellulolytic and beta-glucosidase activities after gel filtration on Sephadex G-75 showed the presence of several active peaks. Glucose was the only product of hydrolysis when native cellulose was used as the substrate. Acid-swollen cellulose, when treated with cellulase free of beta-glucosidase activity, gave rise to glucose, cellobiose, and at least two higher molecular weight components which were also hydrolyzed in turn to cellobiose and glucose. The presence of a multiple cellulolytic enzyme system was indicated, the components of which may have specific roles in the degradation of cellulose.
微小毛霉NRRL 2543的培养滤液含有能作用于天然纤维素、酸溶纤维素、羧甲基纤维素和纤维二糖的水解酶。在Sephadex G - 75上进行凝胶过滤后，纤维素酶和β - 葡萄糖苷酶活性的分布图谱显示有几个活性峰。以天然纤维素为底物时，葡萄糖是水解的唯一产物。用不含β - 葡萄糖苷酶活性的纤维素酶处理酸溶纤维素时，产生了葡萄糖、纤维二糖以及至少两种较高分子量的成分，这些成分又依次水解为纤维二糖和葡萄糖。这表明存在一个多纤维素酶系统，其组分在纤维素降解过程中可能具有特定作用。
