淀粉样蛋白诊断组织化学现有方法的评估。
A n evaluation of current methods for the diagnostic histochemistry of amyloid.
作者信息
Cooper J H
出版信息
J Clin Pathol. 1969 Jul;22(4):410-3. doi: 10.1136/jcp.22.4.410.
Six current histological methods for demonstrating amyloid (crystal violet, thioflavine-T fluorescence, Congo-red staining and fluorescence, Sirius-red staining, and Congo- or Sirius-red birefringence) were applied in 25 cases of amyloidosis of various types and 47 pseudo-amyloid lesions. The results were compared and were correlated with those of ancillary histochemical tests and clinico-pathological data and each method's sensitivity and specificity for amyloid was evaluated. Thioflavine-T and, to a lesser degree, Congo-red fluorescence and Sirius-red staining proved very sensitive but not specific. Green birefringence with Congo or Sirius red was specific but not completely sensitive. The coexistence of Congo-red (and Sirius-red) staining and a positive DMAB-nitrite reaction occurred in all amyloid specimens and appeared specific for amyloid.
六种目前用于显示淀粉样蛋白的组织学方法（结晶紫法、硫黄素-T荧光法、刚果红染色及荧光法、天狼星红染色法以及刚果红或天狼星红双折射法）应用于25例不同类型的淀粉样变性病例及47例假淀粉样病变。对结果进行了比较，并与辅助组织化学检测结果及临床病理数据相关联，评估了每种方法对淀粉样蛋白的敏感性和特异性。硫黄素-T以及程度稍低的刚果红荧光和天狼星红染色证明非常敏感但不具有特异性。刚果红或天狼星红染色后的绿色双折射具有特异性但并非完全敏感。刚果红（和天狼星红）染色与阳性二甲基氨基联苯-亚硝酸盐反应同时存在于所有淀粉样蛋白标本中，似乎对淀粉样蛋白具有特异性。
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