Wen Y F
Zhonghua Min Guo Wei Sheng Wu Xue Za Zhi. 1977 Sep;10(3):74-9.
Twenty-four Achatina fulica from Pai-sha of the Pescardores Islands, where no Angiostrogylus cantonensis has been found in A. fulica(4), were inoculated with the first stage larvae of A cantonensis. One snail was killed every day after 24 days following inoculation. The lung of each snail was separated and examined for larvae under microscope. The same lung was then digested with pepsin. The larvae recovered after pepsin digestion were examined for more detailed morphology. This study confirmed that the first stage larvae of A. cantonensis passed through the respiratory pore to the lung of A. fulica and developed into infectious form (3rd stage larvae) in the lung. The second and third stage larvae were found in the lung on the 7th and 17th day respectively after inoculation. Worm capsules were found in the lung on the 12th day after inoculation and the size of capsule was in proportion to the duration of the infection.
从澎湖白沙采集的24只褐云玛瑙螺(在该地区褐云玛瑙螺中未发现广州管圆线虫(4))接种广州管圆线虫一期幼虫。接种后24天,每天处死1只螺。分离每只螺的肺脏,在显微镜下检查幼虫。然后用胃蛋白酶消化同一肺脏。对经胃蛋白酶消化后回收的幼虫进行更详细的形态学检查。本研究证实,广州管圆线虫一期幼虫通过呼吸孔进入褐云玛瑙螺肺脏,并在肺脏中发育为感染性形态(三期幼虫)。接种后第7天和第17天分别在肺脏中发现二期和三期幼虫。接种后第12天在肺脏中发现虫囊,且虫囊大小与感染持续时间成正比。