染色体检查结果对淋巴瘤与淋巴母细胞系鉴别诊断的意义。

The significance of chromosomal findings for the differentiation between lymphoma and lymphoblastoid cell lines.

作者信息

Hellriegel K P, Diehl V, Krause P H, Meier S, Blankenstein M, Busche W

出版信息

Haematol Blood Transfus. 1977;20:307-13. doi: 10.1007/978-3-642-66639-1_38.

Abstract

Cytogenetic studies have been performed from four Burkitt's lymphoma cell lines, one cell line obtained after successful xenotransplantation of a Burkitt's lymphoma cell line into athymic nude mice, and from ten lymphoblastoid cell lines (three derived from umbilical cord blood cells, one from acute myelogenous leukemia and six from Hodgkin's disease specimens). Our findings indicate the non-neoplastic nature of lymphoblastoid cell lines in spite of their high proliferative activity in vitro and their aneuploidy after long-term cultivation. In contrast, cell lines of Burkitt's lymphoma origin apparently are truly malignant lymphoma cells, characterized by aneuploidy and by the presence of a 14q+-marker chromosome even in the EBV-negative cell lines. The 14q+-marker chromosome, observed in Burkitt's lymphoma as well as in other malignant lymphomas, seems to be related to neoplasia, independent on the presence of EBV. The significance of the aneuploidy for the differentiation between lymphoma and lymphoblastoid cell lines is discussed.

摘要

对4个伯基特淋巴瘤细胞系、1个在成功将伯基特淋巴瘤细胞系异种移植到无胸腺裸鼠后获得的细胞系以及10个淋巴母细胞系（3个源自脐带血细胞，1个源自急性髓性白血病，6个源自霍奇金病标本）进行了细胞遗传学研究。我们的研究结果表明，淋巴母细胞系尽管在体外具有高增殖活性且长期培养后为非整倍体，但仍具有非肿瘤性质。相比之下，源自伯基特淋巴瘤的细胞系显然是真正的恶性淋巴瘤细胞，其特征为非整倍体，甚至在EBV阴性的细胞系中也存在14q +标记染色体。在伯基特淋巴瘤以及其他恶性淋巴瘤中观察到的14q +标记染色体似乎与肿瘤形成有关，与EBV的存在无关。讨论了非整倍体在淋巴瘤和淋巴母细胞系鉴别中的意义。