Ting C C, Bonnard G D
J Immunol. 1976 May;116(5):1419-25.
Primary and secondary cell-mediated cytotoxic responses to FBL-3 cells, a syngeneic Friend virus-induced leukemia in C57BL/6 mice, could be generated by in vitro techniques as tested by the 125IUdR release assay. The specificity of the cytotoxic reactions appeared to be directed against the Friend type-specific antigen and the FMR (Friend, Moloney, Rauscher) antigen which were also the major antigens for transplantation immunity to FBL-3. In comparison to the primary cytotoxic response, the secondary cytotoxic response was accelerated (detected at an earlier time after sensitization), enhanced (gave much higher levels of cytotoxicity), was also longer lasting, and could be induced by a wide dose range of tumor cells. The secondary response could only be induced with lymphocytes obtained from regressors that were resistant to FBL-3 challenge; lymphocytes from mice with progressive tumor growth had no detectable secondary response. It was found that both induction phase and the effector phase of cytotoxic responses were T cell dependent. The characteristics of these reactions were thus very similar to those obtained with in vivo immunization or challenge, providing a good correlation with in vivo tumor immunity.
通过125IUdR释放试验检测发现，采用体外技术可产生对FBL-3细胞（一种C57BL/6小鼠同基因Friend病毒诱导的白血病细胞）的原发性和继发性细胞介导的细胞毒性反应。细胞毒性反应的特异性似乎针对Friend型特异性抗原和FMR（Friend、Moloney、Rauscher）抗原，这些也是对FBL-3移植免疫的主要抗原。与原发性细胞毒性反应相比，继发性细胞毒性反应加速（致敏后更早时间检测到）、增强（产生更高水平的细胞毒性）、持续时间也更长，并且可由广泛剂量范围的肿瘤细胞诱导。继发性反应只能用从对FBL-3攻击有抗性的消退小鼠获得的淋巴细胞诱导；肿瘤进行性生长的小鼠的淋巴细胞没有可检测到的继发性反应。发现细胞毒性反应的诱导阶段和效应阶段均依赖于T细胞。因此，这些反应的特征与体内免疫或攻击所获得的特征非常相似，与体内肿瘤免疫具有良好的相关性。
