Sulitzeanu D, Birnbaum M
Immunology. 1969 Aug;17(2):181-7.
Mice were primed by footpad inoculation of small amounts of BSA (0.1–2.5 μg) in incomplete Freund's adjuvant. Lymphoid cells removed from these mice 50–120 days after priming were cultured in diffusion chambers, either in the presence of BSA or following a brief period of incubation with BSA . Small but clearly measurable amounts of anti-BSA antibody were produced by cells stimulated with amounts of antigen in the range 0.1–10 μg. The optimum boosting dose seemed to be related to the priming dose. The system described appears to be eminently suitable for the autoradiographic follow up of the interaction of cells with antigen in the secondary response.
通过在不完全弗氏佐剂中足垫接种少量牛血清白蛋白(0.1 - 2.5μg)对小鼠进行致敏。在致敏后50 - 120天从小鼠体内取出的淋巴细胞在扩散小室中培养,培养环境要么是存在牛血清白蛋白的情况,要么是在与牛血清白蛋白短暂孵育之后。被0.1 - 10μg范围内的抗原量刺激的细胞产生了少量但明显可测量的抗牛血清白蛋白抗体。最佳增强剂量似乎与致敏剂量有关。所描述的这个系统似乎非常适合在二次应答中对细胞与抗原相互作用进行放射自显影追踪。