使用高效液相色谱法测定17β-羟基-7α-甲基雄甾-5-烯-3-酮（RMI 12,936）对大鼠卵巢匀浆产生孕酮的影响。

Use of HPLC to determine the effect of 17 beta-hydroxy-7 alpha-methylandrost-5-en-3-one (RMI 12,936) on production of progesterone by rat ovarian homogenate.

作者信息

Taylor R B, Kendle K E

出版信息

Acta Endocrinol (Copenh). 1978 May;88(1):157-63. doi: 10.1530/acta.0.0880157.

DOI:10.1530/acta.0.0880157

Abstract

A new high presure liquid chromatography (HPLC) method for the determination of progesterone in the presence of other delta 43-ketosteroids is described. Using this method it is shown that the rate of production of progesterone from pregn-5-ene-3,20-dione by rat ovarioan homogenate is initally rapid but falls to zero within 10 min. Experiments indicate that the inhibition is due to the progesterone formed. Inclusion of RMI 12,936 with the pregn-5-ene-3,20-dione substrate results in a lower final level of progesterone and continuous production of 7 alpha-methyltestosterone. The reduction in the levels of progesterone in presence of RMI 12,936 corresponds closely to the reduction in plasma progesterone in vivo folowing administration of RMI 12,936 described previously.

摘要

本文描述了一种新的高效液相色谱（HPLC）方法，用于在存在其他δ4 3-酮类固醇的情况下测定孕酮。使用该方法表明，大鼠卵巢匀浆将孕-5-烯-3,20-二酮转化为孕酮的速率最初很快，但在10分钟内降至零。实验表明，抑制作用是由于形成的孕酮所致。在孕-5-烯-3,20-二酮底物中加入RMI 12,936会导致孕酮的最终水平降低，并持续产生7α-甲基睾酮。在存在RMI 12,936的情况下孕酮水平的降低与先前描述的给予RMI 12,936后体内血浆孕酮的降低密切相关。