Kulangara A C
J Reprod Fertil. 1976 Jan;46(1):189-94. doi: 10.1530/jrf.0.0460189.
Human serum albumin (HSA) and human gamma globulin (HGG) in serum and uterine fluid of nonpregnant rabbits at various times after an i.v. injection (100 mg/kg) were measured by a radial immunodiffusion test using specific antisera. The HSA concentration in uterine fluid rose to a peak at 12 hr when it was 11% of the serum concentration and then declined, whereas HGG reached a peak at 18 hr (3.2% of serum level) and decreased thereafter. The HSA passed 2 1/2 times faster than HGG, but both proteins equilibrated with uterine fluid in about 12-18 hr. Steady state levels of HSA and HGG indicated that uterine fluid: serum ratios were 1:10 and 1:20, respectively. Similar ratios were found for total protein and rabbit serum albumin (1:10) and rabbit gamma globulin (1:20). Therefore, except when there is a local immune response, the uterine lumen contains only about 5% of the serum antibody concentration. Available data in the mouse, rat and dog also indicate disparity between serum and uterine fluid protein levels.
通过使用特异性抗血清的放射免疫扩散试验，测定了静脉注射（100mg/kg）后不同时间非孕兔血清和子宫液中的人血清白蛋白（HSA）和人丙种球蛋白（HGG）。子宫液中HSA浓度在12小时时升至峰值，为血清浓度的11%，随后下降，而HGG在18小时时达到峰值（血清水平的3.2%），此后降低。HSA的通过速度比HGG快2.5倍，但两种蛋白质在约12 - 18小时内与子宫液达到平衡。HSA和HGG的稳态水平表明，子宫液与血清的比例分别为1:10和1:20。总蛋白、兔血清白蛋白（1:10）和兔丙种球蛋白（1:20）也发现了类似比例。因此，除非存在局部免疫反应，否则子宫腔内仅含有约5%的血清抗体浓度。在小鼠、大鼠和狗中的现有数据也表明血清和子宫液蛋白质水平存在差异。
