Brunnert H, Matsumura F
Biochem J. 1969 Aug;114(1):135-9. doi: 10.1042/bj1140135.
DDT (1,1,1-trichloro-2,2-di-p-chlorophenylethane) and DDE (1,1-dichloro-2,2-di-p-chlorophenylethylene, a non-insecticidal analogue of DDT) were found to bind with various nerve components of rat brain. The amount of DDT binding exceeded that of DDE only in the fraction containing mainly the nerve endings. Among various components in the nerve-ending fraction, a subfraction containing mainly the pre- and post-synaptic complexes had the highest affinity for DDT in comparison with that for DDE. By using an ;acetone-powdering' technique on brain preparations, the Sephadex-column method was shown to provide reliable results for studies on the binding of DDT or DDE with soluble proteins in the nerve tissues. From these results it was concluded that DDE had a higher affinity for soluble components of the rat brain than did DDT.
人们发现，滴滴涕（1,1,1-三氯-2,2-二对氯苯基乙烷）和滴滴伊（1,1-二氯-2,2-二对氯苯基乙烯，滴滴涕的一种非杀虫类似物）能与大鼠脑的各种神经成分结合。仅在主要包含神经末梢的部分中，滴滴涕的结合量超过了滴滴伊。在神经末梢部分的各种成分中，主要包含突触前和突触后复合体的一个亚组分对滴滴涕的亲和力高于对滴滴伊的亲和力。通过对脑制剂采用“丙酮粉化”技术，结果表明，葡聚糖凝胶柱法为研究滴滴涕或滴滴伊与神经组织中可溶性蛋白质的结合提供了可靠的结果。从这些结果可以得出结论，滴滴伊对大鼠脑可溶性成分的亲和力高于滴滴涕。
