泌乳大鼠和兔乳腺中的丙酮酸羧化酶。
Pyruvate carboxylase in lactating rat and rabbit mammary gland.
作者信息
Gul B, Dils R
出版信息
Biochem J. 1969 Feb;111(3):263-71. doi: 10.1042/bj1110263.
Abstract
- Pyruvate carboxylase [pyruvate-carbon dioxide ligase (ADP), EC 6.4.1.1] was found in cell-free preparations of lactating rat and rabbit mammary glands, and optimum assay conditions for this enzyme were determined. 2. Subcellular-fractionation studies with marker enzymes showed pyruvate carboxylase to be distributed between the mitochondrial and soluble fractions of lactating rat mammary gland. Evidence is presented that the soluble enzyme is not an artifact due to mitochondrial damage. 3. In contrast, pyruvate carboxylase in lactating rabbit mammary gland is confined to the mitochondrial fraction. 4. The final product of pyruvate carboxylase action in the mitochondrial and particle-free supernatant fractions of lactating rat mammary gland was shown to be citrate. 5. The effects of freeze-drying, ultrasonic treatment and freezing-and-thawing on the specific activity of mitochondrial pyruvate carboxylase were investigated.
摘要
- 在泌乳大鼠和家兔乳腺的无细胞制剂中发现了丙酮酸羧化酶[丙酮酸-二氧化碳连接酶（ADP），EC 6.4.1.1]，并确定了该酶的最佳测定条件。2. 用标记酶进行的亚细胞分级分离研究表明，丙酮酸羧化酶分布于泌乳大鼠乳腺的线粒体和可溶性部分。有证据表明，可溶性酶并非线粒体损伤所致的假象。3. 相比之下，泌乳家兔乳腺中的丙酮酸羧化酶局限于线粒体部分。4. 已证明泌乳大鼠乳腺线粒体和无颗粒上清液部分中丙酮酸羧化酶作用的终产物是柠檬酸。5. 研究了冷冻干燥、超声处理和冻融对线粒体丙酮酸羧化酶比活性的影响。
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