Pengra R M, Cole M A, Alexander M
J Bacteriol. 1969 Mar;97(3):1056-61. doi: 10.1128/jb.97.3.1056-1061.1969.
Walls of Mortierella parvispora, Pullularia pullulans, Absidia repens, Fusarium oxysporum, and of several Penicillium species varied in their susceptibilities to digestion by glucanase and chitinase. Polysaccharides were present in the residues remaining after enzymatic digestion. Acid hydrolysates of the walls contained glucose, glucosamine, and a small amount of galactose. The walls of M. parvispora, which also contained fucose, were the least digested by these two enzymes. Much of the M. parvispora wall material was resistant to decomposition by a heterogeneous soil community, and viable hyphae were not lysed by a glucanase-chitinase mixture. Walls of this fungus were fractionated, and the chemical composition of the fractions was determined. The chitin which was abundant in one of the fractions was apparently largely shielded from chitinase hydrolysis by a glucan. The ecological significance of these findings is discussed.
小孢被孢霉、出芽短梗霉、匍匐犁头霉、尖孢镰刀菌以及几种青霉的细胞壁对葡聚糖酶和几丁质酶消化的敏感性各不相同。酶消化后残留的物质中存在多糖。细胞壁的酸水解产物含有葡萄糖、氨基葡萄糖和少量半乳糖。同样含有岩藻糖的小孢被孢霉的细胞壁对这两种酶的消化作用最不敏感。小孢被孢霉的许多细胞壁物质对异质土壤群落的分解具有抗性，并且活菌丝不会被葡聚糖酶 - 几丁质酶混合物溶解。对这种真菌的细胞壁进行了分级分离，并测定了各组分的化学成分。其中一个组分中大量存在的几丁质显然在很大程度上被一种葡聚糖保护而免受几丁质酶的水解。讨论了这些发现的生态学意义。
