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转移的肺表面活性物质膜:化学分析与接触角研究

Transferred pulmonary surfactant film: chemical analysis and contact angle study.

作者信息

Lee L P, Lee J S

出版信息

J Bioeng. 1978 Jun;2(3-4):259-67.

PMID:581383
Abstract

The Langmuir dip plate transfer technique was adapted to isolate the interfacial film formed at the surface of lung wash. The chemical analysis of the film showed that the weight ratio of lipid to protein of this surfactant film and its content of surface active substances were higher than those of lung wash. It was found that the wettability of the surfactant film could be simulated by a Dipalmitoyl lecithin and albumin film. When the surfactant film was transferred at condensed state (one of low air-lung-wash surface tension), we found the film exhibited a low critical surface tension which indicates the dominance of the lipid in the outer most layer of the film. On the other hand, insignificant amount of lipid was found in the film transferred at expanded state (one of high surface tension) and its surface behavior was shown to be similar to that of a protein film.

摘要

采用朗缪尔浸盘转移技术来分离肺灌洗表面形成的界面膜。对该膜的化学分析表明,这种表面活性剂膜的脂质与蛋白质重量比及其表面活性物质含量高于肺灌洗。研究发现,二棕榈酰卵磷脂和白蛋白膜可模拟表面活性剂膜的润湿性。当表面活性剂膜以凝聚态(低气-肺-灌洗表面张力之一)转移时,我们发现该膜表现出较低的临界表面张力,这表明脂质在膜的最外层占主导地位。另一方面,在以膨胀态(高表面张力之一)转移的膜中发现脂质含量极少,其表面行为与蛋白质膜相似。

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