MacKintosh F R, Bell E
Science. 1969 May 23;164(3882):961-3. doi: 10.1126/science.164.3882.961.
Acrylamide-gel patterns of proteins made before and during the first 30 minutes after fertilization were the same. The patterns of gels containing proteins from eggs whose rate of protein synthesis was stimulated reversibly by prior anaerobiosis or removal of carbon dioxide were also the same as that of the mature unfertilized egg. Changes were detected in the pattern of proteins synthesized in gastrulas as compared to proteins made in unfertilized eggs or zygotes.
受精前及受精后头30分钟内所产生蛋白质的丙烯酰胺凝胶图谱是相同的。含有经前期厌氧处理或去除二氧化碳而使其蛋白质合成速率受到可逆性刺激的卵子中蛋白质的凝胶图谱,也与未受精成熟卵子的凝胶图谱相同。与未受精卵或受精卵中所产生的蛋白质相比,原肠胚中合成的蛋白质图谱出现了变化。