纯化猪胰弹性蛋白酶的特异性
The specificity of purified porcine pancreatic elastase.
作者信息
Narayanan A S, Anwar R A
出版信息
Biochem J. 1969 Aug;114(1):11-7. doi: 10.1042/bj1140011.
Abstract
An electrophoretically homogeneous elastase preparation free from tryptic and chymotryptic activities was obtained by chromatography on DEAE-Sephadex and CM-cellulose. This preparation exhibits a narrower specificity towards peptide bonds than that observed by Naughton & Sanger (1961). With oxidized insulin B chain as substrate, the fastest breaks occur between alanine-14 and leucine-15 and between valine-18 and cysteic acid-19. The bond between glycine-23 and phenylalanine-24 is also efficiently hydrolysed. Other bonds hydrolysed are that between valine-12 and glutamic acid-13 and that between serine-9 and histidine-10. Oxidized insulin A chain is hydrolysed only at one of two points, between alanine-8 and serine-9 or between serine-12 and leucine-13, and the rate of hydrolysis is very low.
摘要
通过在DEAE-葡聚糖凝胶和CM-纤维素上进行色谱分离，获得了一种无胰蛋白酶和糜蛋白酶活性的电泳纯弹性蛋白酶制剂。该制剂对肽键的特异性比诺顿和桑格（1961年）观察到的更窄。以氧化胰岛素B链为底物时，最快的断裂发生在丙氨酸-14和亮氨酸-15之间以及缬氨酸-18和半胱氨酸-19之间。甘氨酸-23和苯丙氨酸-24之间的键也能被有效水解。其他被水解的键是缬氨酸-12和谷氨酸-13之间的键以及丝氨酸-9和组氨酸-10之间的键。氧化胰岛素A链仅在两个点之一被水解，即在丙氨酸-8和丝氨酸-9之间或丝氨酸-12和亮氨酸-13之间，且水解速率非常低。
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本文引用的文献
1
Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.碱性蛋白质和肽在聚丙烯酰胺凝胶上的圆盘电泳。
Nature. 1962 Jul 21;195:281-3. doi: 10.1038/195281a0.
2
The use of cyanate for the determination of NH2-terminal residues in proteins.用氰酸盐测定蛋白质中的氨基末端残基。
J Biol Chem. 1963 Jan;238:214-26.
3
Zone electrophoresis in starch gels and its application to studies of serum proteins.淀粉凝胶区带电泳及其在血清蛋白研究中的应用。
Adv Protein Chem. 1959;14:65-113. doi: 10.1016/s0065-3233(08)60609-9.
4
A modified procedure for the automatic analysis of amino acids.一种用于氨基酸自动分析的改进方法。
Anal Biochem. 1960 Nov;1:187-201. doi: 10.1016/0003-2697(60)90045-2.
5
Purification and specificity of pancreatic elastase.胰腺弹性蛋白酶的纯化及特异性
Biochem J. 1961 Jan;78(1):156-63. doi: 10.1042/bj0780156.
6
Pancreatic elastase: purification, properties, and function.胰腺弹性蛋白酶：纯化、性质及功能
J Biol Chem. 1956 Oct;222(2):705-20.
7
A colorimetric procedure for the quantitative determination of tryptophan residues in proteins.一种用于定量测定蛋白质中色氨酸残基的比色法。
J Biol Chem. 1967 Dec 25;242(23):5771-6.
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10
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