Smith B T
Science. 1979 Jun 8;204(4397):1094-5. doi: 10.1126/science.582216.
Fibroblast-pneumonocyte factor, produced by the fetal lung fibroblast in response to glucocorticoids, was partially purified by column chromatography on Sephadex G-75. The resulting preparation showed two major and two minor bands on sodium dodecylsulfate-polyacrylamide gel electrophoresis. When fetal rats were injected on day 17 of gestation with 1 microgram of this material, they showed on day 20 biochemical evidence of accelerated lung maturation as compared to littermate controls. There were no differences between the two groups in body weights, organ weights, or circulating corticosteroid levels.
成纤维细胞 - 肺上皮细胞因子由胎儿肺成纤维细胞在糖皮质激素作用下产生，通过在葡聚糖凝胶G - 75上进行柱色谱法进行部分纯化。所得制剂在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳上显示出两条主要条带和两条次要条带。当在妊娠第17天给胎鼠注射1微克这种物质时，与同窝对照相比，它们在第20天显示出肺成熟加速的生化证据。两组在体重、器官重量或循环皮质类固醇水平方面没有差异。
