Robison W L, Davis D
J Cell Biol. 1969 Oct;43(1):115-21. doi: 10.1083/jcb.43.1.115.
The concentration and the distribution of iodine in various sized follicles of rat thyroid glands have been analyzed by electron-probe microanalysis. The results of the iodine analysis were grouped according to uncorrected lumen diameter size. No significant differences in iodine concentration were observed among the various size categories. When the results for all follicles from a given sample were pooled, the standard error of the mean was approximately 4%. Usually 40-50 follicles per animal were analyzed. The concentration of iodine ranged from 0.9 to 2.1% by weight among individual animals. Scanning pictures and step-scan analysis showed the iodine distribution to be quite uniform across the colloid area. Several techniques of sample preparation were used; they produced no significant differences in the iodine concentrations observed. Sodium concentration, also determined in all samples, was found to vary from 2 to 9% by weight. Because of the mobility of the sodium ion, its distribution was greatly affected by the method of sample preparation. The technique that best preserved the natural chemistry of the sample was that of freezing the tissue, sectioning, and then freeze-drying.
利用电子探针微量分析技术，对大鼠甲状腺不同大小滤泡中的碘浓度及分布情况进行了分析。碘分析结果按未校正的管腔直径大小分组。不同大小类别的滤泡之间未观察到碘浓度有显著差异。当将给定样本中所有滤泡的结果汇总时，平均标准误差约为4%。通常每只动物分析40 - 50个滤泡。个体动物中碘的浓度按重量计在0.9%至2.1%之间。扫描图片和步进扫描分析表明，碘在胶体区域的分布相当均匀。使用了几种样本制备技术；它们在观察到的碘浓度上没有产生显著差异。在所有样本中也测定了钠浓度，发现其按重量计在2%至9%之间变化。由于钠离子的流动性，其分布受样本制备方法的影响很大。最能保留样本自然化学性质的技术是将组织冷冻、切片，然后冷冻干燥。
