Kuhnert P M, Erhard P, Kuhnert B R, Sokol R J, Gross T L
Am J Obstet Gynecol. 1979 Oct 1;135(3):331-6. doi: 10.1016/0002-9378(79)90700-2.
Several desirable techniques (rapid chromatogram development, planimetry, acetone precipitation of lecithin, and copper molybdate staining) used in other published lecithin/sphingomyelin (L/S) ratio procedures were integrated into a single L/S ratio test. The resulting test requires only 2 ml of amniotic fluid, can be performed within 75 minutes, and is semiquantitative. Methodology tests showed a high degree of reproducibility without the need for a densitometer: Coefficients of variation for the standards and amniotic fluid samples were 11% and 4%, respectively. Also, a linear relationship was observed between the L/S weight ratios in synthetic mixtures and the corresponding area ratios up to the mature value of 2.5. Clinical evaluation on a normal and high-risk patient population showed excellent reliability: The accuracy in predicting fetal lung maturity and immaturity was 100% and 85%, respectively. Moreover, the numerical value of the L/S ratio in the immature range was found to be indicative of the severity of respiratory distress syndrome. Finally, the relationship between the L/S ratio and gestational age in a normal population was described mathematically by an approximating curve. We concluded from our methodologic and clinical data that the L/S ratio may be determined simply and reliably by means of the procedure described in this report.
其他已发表的卵磷脂/鞘磷脂（L/S）比值检测方法中使用的几种理想技术（快速色谱展开、平面测量法、卵磷脂的丙酮沉淀法和钼酸铜染色法）被整合到一个单一的L/S比值检测中。由此产生的检测方法仅需2毫升羊水，可在75分钟内完成，并且是半定量的。方法学测试表明，该方法具有高度的可重复性，无需使用密度计：标准品和羊水样本的变异系数分别为11%和4%。此外，在合成混合物中，L/S重量比与相应面积比之间在成熟值2.5之前呈现线性关系。对正常和高危患者群体的临床评估显示出极佳的可靠性：预测胎儿肺成熟和不成熟的准确率分别为100%和85%。此外，发现在不成熟范围内L/S比值的数值可指示呼吸窘迫综合征的严重程度。最后，通过一条近似曲线以数学方式描述了正常人群中L/S比值与胎龄之间的关系。基于我们的方法学和临床数据，我们得出结论，L/S比值可以通过本报告中描述的方法简单而可靠地测定。
