DNA：与氯喹的反应
DNA: reaction with chloroquine.
作者信息
Allison J L, O'Brien R L, Hahn F E
出版信息
Science. 1965 Sep 3;149(3688):1111-3. doi: 10.1126/science.149.3688.1111.
PMID:5826527
Abstract
Diflerence spectrophotometry shows that double-stranded DNA produces mnarked changes in the absorption spectrum of chloroquine; only minor changes occur with single-stranded DNA. A DNA-chloroquine complex was demonstrated to sediment in the analytical ultracentrifuge. Chloroquine strongly elevated the thermal dissociation temperature, T(m), of DNA. It is concluded that the drug forms a complex with DNA by ionic interaction and stabilizes the helix.
摘要
差示分光光度法表明，双链DNA会使氯喹的吸收光谱发生显著变化；而单链DNA只会引起微小变化。已证实在分析超速离心机中DNA - 氯喹复合物会发生沉降。氯喹能显著提高DNA的热解链温度T(m)。结论是该药物通过离子相互作用与DNA形成复合物并使螺旋结构稳定。
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