Hentschel H
Tissue Cell. 1979;11(3):517-31. doi: 10.1016/0040-8166(79)90060-0.
Kidney cells of the marine stickleback Spinachia have been studied with histochemical methods for the demonstration of glycoconjugates. The fine structure of epithelial cells is described. Mucus threads in the nephronic tubule of sexually mature consist of neutral glycoprotein which corresponds with the secretory granules in proximal tubule segment II cells. Large lysosome-like inclusions, which also react with PAS, are present in many P II cells. All cells of the collecting duct epithelium differentiate into mucous cells in male Spinachia. The nature of their secretory products, which are well preserved by freeze-drying, is discussed. Sialylated glycoprotein is present in mucus granules and sulphated glycoprotein can be demonstrated at the apex of collecting duct cells. Collecting duct cell mucus can be digested with testicular hyaluronidase indicating that proteoglycans may be involved in the structure of macromolecules. The observations are compared with studies of mucus production in the urinary apparatus of several other vertebrates.
已采用组织化学方法对海刺鱼Spinachia的肾细胞进行研究，以显示糖缀合物。文中描述了上皮细胞的精细结构。性成熟的刺鱼肾小管中的黏液丝由中性糖蛋白组成，这与近端小管II段细胞中的分泌颗粒相对应。许多近端小管II段细胞中存在大型溶酶体样内含物，这些内含物也与PAS反应。在雄性Spinachia中，集合管上皮的所有细胞都分化为黏液细胞。文中讨论了通过冷冻干燥保存良好的其分泌产物的性质。黏液颗粒中存在唾液酸化糖蛋白，并且在集合管细胞顶端可显示硫酸化糖蛋白。集合管细胞黏液可被睾丸透明质酸酶消化，表明蛋白聚糖可能参与大分子的结构。将这些观察结果与其他几种脊椎动物泌尿器官中黏液产生的研究进行了比较。
