5-氟尿嘧啶对噬菌体R17生长的影响。
Effect of 5-fluorouracil on the growth of bacteriophage R17.
作者信息
Graham A F, Kirk C
出版信息
J Bacteriol. 1965 Oct;90(4):928-35. doi: 10.1128/jb.90.4.928-935.1965.
Graham, A. F. (The Wistar Institute of Anatomy and Biology, Philadelphia, Pa.), and Clare Kirk. Effect of 5-fluorouracil on the growth of bacteriophage R17. J. Bacteriol. 90:928-935. 1965.-When added to Escherichia coli within 2 min after phage R17, 5-fluorouracil (FU), at a concentration of 10(-4)m, completely inhibited the synthesis of infectious ribonucleic acid (RNA) and phage. If the addition of FU was made later than 5 min after infection, infectious RNA synthesis was blocked but infectious phage was still formed; the infectious RNA made before the addition of FU continued to be incorporated into mature phage. These properties of the inhibitor were used to determine the kinetics of phage RNA synthesis and the size of the phage precursor RNA pool. At a concentration of 2.2 x 10(-5)m FU, the yield of phage was reduced to 15% of that in an uninhibited control, 28% of the phage RNA uracil was replaced with FU, and the specific infectivity of the phage was unaltered.
格雷厄姆，A.F.（宾夕法尼亚州费城威斯塔研究所解剖学与生物学部），以及克莱尔·柯克。5-氟尿嘧啶对噬菌体R17生长的影响。《细菌学杂志》90:928 - 935。1965年。——在噬菌体R17感染大肠杆菌后2分钟内加入浓度为10⁻⁴摩尔的5-氟尿嘧啶（FU），可完全抑制感染性核糖核酸（RNA）和噬菌体的合成。如果在感染后5分钟后加入FU，感染性RNA合成受阻，但仍会形成感染性噬菌体；在加入FU之前合成的感染性RNA会继续整合到成熟噬菌体中。利用抑制剂的这些特性来确定噬菌体RNA合成的动力学以及噬菌体前体RNA池的大小。在浓度为2.2×10⁻⁵摩尔的FU作用下，噬菌体产量降至未受抑制对照的15%，28%的噬菌体RNA尿嘧啶被FU取代，且噬菌体的比感染性未改变。
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