Anderson J I, Heffernan W P
J Bacteriol. 1965 Dec;90(6):1713-8. doi: 10.1128/jb.90.6.1713-1718.1965.
Anderson, J. I. W. (Northeast Shellfish Sanitation Research Center, Narragansett, R.I.), and W. P. Heffernan. Isolation and characterization of filterable marine bacteria. J. Bacteriol 90:1713-1718. 1965.-By a process of double filtration of seawater, first through a membrane filter with a pore diameter of 0.45 mu and then through a membrane filter with a pore diameter of 0.22 mu, it was possible to isolate on the surface of the latter membrane a group of marine organisms not usually encountered by conventional techniques of pour plates or one-stage filtration. Many of the isolates could not be identified, but the largest single group belonged to the genus Spirillum; other isolates were placed in the genera Leucothrix, Flavobacterium, Cytophaga, and Vibrio. A group of four organisms which was not identified was characterized by the formation of large, club-shaped cells, 20 to 30 mu long. Of the 25 strains studied in detail, 22 required seawater for growth and 8 retained their filterable property after cultivation. No filterable bacteria were isolated from terrestrial samples.
安德森，J. I. W.（罗德岛州纳拉甘西特东北贝类卫生研究中心）和W. P. 赫弗南。可过滤海洋细菌的分离与特性研究。《细菌学杂志》90:1713 - 1718。1965年。——通过对海水进行双重过滤的过程，首先通过孔径为0.45微米的膜过滤器，然后通过孔径为0.22微米的膜过滤器，能够在后一种膜的表面分离出一组通常用倾注平板法或单级过滤的传统技术无法遇到的海洋生物。许多分离物无法鉴定，但最大的单一菌群属于螺菌属；其他分离物被归入亮发菌属、黄杆菌属、噬纤维菌属和弧菌属。有一组四种未鉴定的生物，其特征是形成长20至30微米的大型棒状细胞。在详细研究的25个菌株中，22个菌株生长需要海水，8个菌株培养后仍保持其可过滤特性。未从陆地样品中分离出可过滤细菌。
