Szentirmai A, Szeleczky Z, Tömorkény E
Acta Microbiol Acad Sci Hung. 1975;22(4):463-71.
Yeasts can advantageously be utilized for the production of the 17beta-hydroxy-derivative, from 3-methoxy-8,14-seco-1,3,5(10),9(11)-estratetraene-14,17-dione (14,17-dione) while 14alpha-hydroxy and 14alpha,17beta-dihydroxy-derivatives are also formed. The biochemical properties of yeasts' enzymes responsible for the formation of the two monohydroxy-derivatives have been studied in detail. In the cell-free extract of Saccharomyces the presence of two hydroxysteroid oxidoreductases could be detected. The first enzyme forms 3beta,17beta-dihydroxy-derivative from 5alpha-androstane-317-dione. This enzyme is responsible for the formation of 17beta-hydroxy-derivative from 14,17-dione. The second enzyme forms 3alpha-hydroxy-derivative from 5beta-androstanedione as well as 14alpha-hydroxy-derivative from its 14,17-dione. The cofactor of both enzymes is pyridine nucleotide. The two enzymes possessing different properties can selectively be inhibited.
酵母可有效地用于从3-甲氧基-8,14-开环-1,3,5(10),9(11)-雌甾四烯-14,17-二酮（14,17-二酮）生产17β-羟基衍生物，同时也会形成14α-羟基和14α,17β-二羟基衍生物。对负责形成这两种单羟基衍生物的酵母酶的生化特性进行了详细研究。在酿酒酵母的无细胞提取物中可检测到两种羟基类固醇氧化还原酶。第一种酶从5α-雄甾烷-3,17-二酮形成3β,17β-二羟基衍生物。该酶负责从14,17-二酮形成17β-羟基衍生物。第二种酶从5β-雄甾烷二酮形成3α-羟基衍生物，以及从其14,17-二酮形成14α-羟基衍生物。两种酶的辅因子都是吡啶核苷酸。具有不同特性的两种酶可被选择性抑制。
