植物乳杆菌中的L-谷氨酸-乙醛酸转氨酶
L-Glutamate-glyoxylate aminotransferase in Lactobacillus plantarum.
作者信息
Galas E, Florianowicz T
出版信息
Acta Microbiol Pol B. 1975;7(4):243-52.
Glutamate-glyoxylate aminotransferase which mediates the reaction of glyoxylic acid with glutamic acid to yield glycine and alpha-oxoglutaric acid has been isolated and purified 84-fold from extracts of Lactobacillus plantarum. Purified enzyme requires the addition of pyridoxal phosphate and magnesium ions for its activity. The molecular weight of the enzyme estimated by Sepharose 4B gel filtration amounts to 37.000. Micaelis constants for glyoxylate and glutamate are corresponding to 6.25 X 10(-3) M and 2.75 X 10(-3) M, respectively. Optimal pH in phosphate and veronal buffers is 8.0 and optimal temperature 35--37 degrees C.
谷氨酸-乙醛酸转氨酶可介导乙醛酸与谷氨酸反应生成甘氨酸和α-酮戊二酸，已从植物乳杆菌提取物中分离并纯化了84倍。纯化后的酶需要添加磷酸吡哆醛和镁离子才能发挥活性。通过琼脂糖4B凝胶过滤估计该酶的分子量为37000。乙醛酸和谷氨酸的米氏常数分别对应于6.25×10⁻³ M和2.75×10⁻³ M。在磷酸盐和巴比妥缓冲液中的最适pH为8.0，最适温度为35-37℃。
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