水溶液中¹⁴C标记物质的高效液相闪烁计数及标记蛋白质比活度的测定。
High-efficiency liquid-scintillation counting of 14C-labelled material in aqueous solution and determination of specific activity of labelled proteins.
作者信息
Hall T C, Cocking E C
出版信息
Biochem J. 1965 Sep;96(3):626-33. doi: 10.1042/bj0960626.
Abstract
- Inexpensive scintillation mixtures are described which enable the detection of as little as 40mumuc of (14)C in aqueous solution with an efficiency of counting of over 80%. 2. A rapid method for the counting of alkaline, acidic and neutral aqueous solutions of up to 1ml. volume is described. Ethanol or 2-ethoxyethanol is used as blending agent. 3. The scintillation counting of alkaline solutions is applied to the accurate determination of the specific activity of (14)C-labelled proteins from plant tissues. 4. Attention has been paid to the importance of a standardized washing procedure for the removal of all traces of radioactive material from glassware.
摘要
- 描述了一种廉价的闪烁混合液，它能检测水溶液中低至40毫微居里的（14）C，计数效率超过80%。2. 描述了一种快速计数方法，用于计数体积达1毫升的碱性、酸性和中性水溶液。乙醇或2-乙氧基乙醇用作混合剂。3. 碱性溶液的闪烁计数法用于准确测定植物组织中（14）C标记蛋白质的比活性。4. 已关注到标准化洗涤程序对于从玻璃器皿中去除所有放射性物质痕迹的重要性。
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