甲醇的微生物氧化。假单胞菌属M27的乙醇脱氢酶。
The microbial oxidation of methanol. The alcohol dehydrogenase of Pseudomonas sp. M27.
作者信息
Anthony C, Zatman L J
出版信息
Biochem J. 1965 Sep;96(3):808-12. doi: 10.1042/bj0960808.
Abstract
- No primary hydrogen acceptor other than phenazine methosulphate has been found for the alcohol dehydrogenase from Pseudomonas sp. M27. 2. None of a wide range of vitamins or cofactors has any effect on the activity of the enzyme. 3. The enzyme is far less sensitive to metal-chelating agents and thiol reagents than are other alcohol dehydrogenases. 4. Methanol is oxidized at least as fast as other alcohols by this enzyme and its well-defined substrate specificity is different from that of other alcohol dehydrogenases. Only primary alcohols are oxidized; the general formula for an oxidizable substrate is R.CH(2).OH, where R may be H or [Formula: see text] 5. Whole organisms oxidize only those alcohols that are oxidized by the isolated enzyme.
摘要
- 对于来自假单胞菌属M27的乙醇脱氢酶，除了硫酸吩嗪甲酯外，尚未发现其他主要氢受体。2. 多种维生素或辅因子对该酶的活性均无任何影响。3. 与其他乙醇脱氢酶相比，该酶对金属螯合剂和硫醇试剂的敏感性要低得多。4. 甲醇被该酶氧化的速度至少与其他醇类一样快，并且其明确的底物特异性与其他乙醇脱氢酶不同。仅伯醇被氧化；可氧化底物的通式为R.CH(2).OH，其中R可以是H或[化学式：见正文] 5. 完整生物体仅氧化那些能被分离出的酶氧化的醇类。
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