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甲醇的微生物氧化。假单胞菌属M27的乙醇脱氢酶。

The microbial oxidation of methanol. The alcohol dehydrogenase of Pseudomonas sp. M27.

作者信息

Anthony C, Zatman L J

出版信息

Biochem J. 1965 Sep;96(3):808-12. doi: 10.1042/bj0960808.

DOI:10.1042/bj0960808
PMID:5862419
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1207221/
Abstract
  1. No primary hydrogen acceptor other than phenazine methosulphate has been found for the alcohol dehydrogenase from Pseudomonas sp. M27. 2. None of a wide range of vitamins or cofactors has any effect on the activity of the enzyme. 3. The enzyme is far less sensitive to metal-chelating agents and thiol reagents than are other alcohol dehydrogenases. 4. Methanol is oxidized at least as fast as other alcohols by this enzyme and its well-defined substrate specificity is different from that of other alcohol dehydrogenases. Only primary alcohols are oxidized; the general formula for an oxidizable substrate is R.CH(2).OH, where R may be H or [Formula: see text] 5. Whole organisms oxidize only those alcohols that are oxidized by the isolated enzyme.
摘要
  1. 对于来自假单胞菌属M27的乙醇脱氢酶，除了硫酸吩嗪甲酯外，尚未发现其他主要氢受体。2. 多种维生素或辅因子对该酶的活性均无任何影响。3. 与其他乙醇脱氢酶相比，该酶对金属螯合剂和硫醇试剂的敏感性要低得多。4. 甲醇被该酶氧化的速度至少与其他醇类一样快，并且其明确的底物特异性与其他乙醇脱氢酶不同。仅伯醇被氧化；可氧化底物的通式为R.CH(2).OH，其中R可以是H或[化学式：见正文] 5. 完整生物体仅氧化那些能被分离出的酶氧化的醇类。

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本文引用的文献

1
OXIDATION OF ALIPHATIC GLYCOLS BY ACETIC ACID BACTERIA.醋酸菌对脂肪族二醇的氧化作用
Bacteriol Rev. 1964 Jun;28(2):164-80.
2
The microbial oxidation of methanol. 1. Isolation and properties of Pseudomonas sp. M27.甲醇的微生物氧化。1. 假单胞菌属M27的分离与特性
Biochem J. 1964 Sep;92(3):609-14. doi: 10.1042/bj0920609.
3
The microbial oxidation of methanol. 2. The methanol-oxidizing enzyme of Pseudomonas sp. M 27.甲醇的微生物氧化。2. 假单胞菌属M 27的甲醇氧化酶。
Biochem J. 1964 Sep;92(3):614-21. doi: 10.1042/bj0920614.
4
Microbial growth on C-1 compounds. 6. Oxidation of methanol, formaldehyde and formate by methanol-grown Pseudomonas AM-1.C-1化合物上的微生物生长。6. 甲醇培养的假单胞菌AM-1对甲醇、甲醛和甲酸的氧化作用
Biochem J. 1964 Nov;93(2):281-90. doi: 10.1042/bj0930281.