牛项韧带肿胀度与pH值的关系。
The swelling of bovine ligamentum nuchae as a function of pH.
作者信息
Jackson D S, Sandberg L B, Cleary E G
出版信息
Biochem J. 1965 Sep;96(3):813-7. doi: 10.1042/bj0960813.
Abstract
- The swelling and solubilization of foetal and adult bovine ligament over a range of pH values has been investigated. 2. Maximum swelling occurs at pH2.7 and pH8.5 for both types of ligament. Abover pH13 complete solubilization occurs. 3. Considerably greater swelling and solubilization occurs with foetal ligament below pH12. 4. The presence of 2m-sodium chloride markedly suppresses both swelling and solubilization. Above 1n-sodium hydroxide actual dehydration occurs and little or no protein is extracted. 5. It is suggested that the intermolecular cross-links in a considerable proportion of foetal elastin are weak and that extraction with aqueous salt solution at pH8.5 would provide optimum conditions for extracting soluble proteins, including soluble elastin if this is present.
摘要
- 研究了胎儿和成年牛韧带在一系列pH值范围内的肿胀和溶解情况。2. 两种类型的韧带在pH2.7和pH8.5时出现最大肿胀。在pH13以上会发生完全溶解。3. 在pH12以下，胎儿韧带的肿胀和溶解程度明显更大。4. 2m氯化钠的存在显著抑制了肿胀和溶解。在1n氢氧化钠以上会发生实际脱水，几乎没有或没有蛋白质被提取出来。5. 有人提出，相当一部分胎儿弹性蛋白中的分子间交联较弱，在pH8.5的盐水溶液中提取将为提取可溶性蛋白质（包括如果存在的可溶性弹性蛋白）提供最佳条件。
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2
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