Smith D S, Treherne J E
J Cell Biol. 1965 Aug;26(2):445-65. doi: 10.1083/jcb.26.2.445.
The distribution of esterase activity in the last abdominal ganglion, the connectives and the cereal nerves of the cockroach Periplaneta americana has been investigated cytochemically. Activity of an unspecific eserine-insensitive esterase (or esterases) has been found in glial elements in these regions of the nerve cord. In addition, sites of cholinesterase (eserine-sensitive) activity have been found in association with (a) the glial sheaths of the axons in the cereal nerves and connectives, (b) the glial folds encapsulating the neuron perikarya in the ganglion, and (c) in localized areas along the membranes of axon branches within the neuropile, often flanked by focal clusters of synaptic vesicles. These results are discussed with particular reference to the previously reported insensitivity of the insect nerve cord to applied acetylcholine, and to the probable existence of a cholinergic synaptic mechanism in the central nervous system of this insect.
利用细胞化学方法研究了美洲大蠊最后腹神经节、神经索和触角神经中酯酶活性的分布。在神经索这些区域的神经胶质细胞中发现了一种非特异性的、对毒扁豆碱不敏感的酯酶的活性。此外，还发现胆碱酯酶（对毒扁豆碱敏感）的活性位点与以下部位有关：（a）触角神经和神经索中轴突的神经胶质鞘；（b）神经节中包裹神经元胞体的神经胶质褶皱；（c）神经纤维网内轴突分支膜的局部区域，这些区域常伴有局部的突触小泡簇。结合先前报道的昆虫神经索对施加的乙酰胆碱不敏感以及该昆虫中枢神经系统中可能存在胆碱能突触机制，对这些结果进行了讨论。
