Ross D W
Cell Tissue Kinet. 1976 Jul;9(4):379-87. doi: 10.1111/j.1365-2184.1976.tb01286.x.
The effects of various chemotherapeutic agents on the volume of Chinese hamster V79 fibroblasts and murine lymphoma L5178Y cells were studied by electronic volume spectroscopy. Cells arrested in the division cycle by a chemotherapeutic block continued to grow in volume resulting in abnormally large cells unable to reduce their volume by cell division. This was observed in cells treated with colcemid, vinblastine, excess thymidine, hydroxyurea, ARA-C, 5-fluorouracil, actinomycin-D and bleomycin, but not with puromycin or cycloheximide. Increase in cell volume of blocked cells was correlated with a decrease in cell survival as measured by clonogenic ability. The results suggest the possibility of volume spectroscopy for a rapid in vitro test to determine tumor sensitivity to chemotherapeutic agents and the in vivo monitoring of response to chemotherapy. Mechanisms for increased cell kill by a second agent acting selectively on enlarged cells are considered.
通过电子体积光谱法研究了各种化疗药物对中国仓鼠V79成纤维细胞和小鼠淋巴瘤L5178Y细胞体积的影响。因化疗阻滞而停滞在分裂周期中的细胞继续增大体积，导致细胞异常大，无法通过细胞分裂减小其体积。在用秋水仙酰胺、长春花碱、过量胸苷、羟基脲、阿糖胞苷、5-氟尿嘧啶、放线菌素-D和博来霉素处理的细胞中观察到了这种情况，但在用嘌呤霉素或环己酰亚胺处理的细胞中未观察到。通过克隆形成能力测定，阻滞细胞的细胞体积增加与细胞存活率降低相关。结果表明，体积光谱法有可能用于快速体外试验，以确定肿瘤对化疗药物的敏感性以及体内化疗反应监测。还考虑了第二种药物选择性作用于增大细胞从而增加细胞杀伤的机制。
