Description of the banding patterns of human chromosomes by acriding orange reverse banding (RFA) and comparison with the Paris banding diagram.

A reference diagram is presented which is comparable to the Paris banding diagram but which was based primarily on chromosomes studied by acridine orange reverse banding. Comparison with the Paris Conference (1971) banding diagram was made. Bands were combined in 13 instances and subdivided in 10 cases. The number of bands was found to depend primarily upon the length and quality of the chromosomes. No major differences between the present diagram and the Paris banding diagram were observed. Moreover, the results with acriding orange reverse banding were comparable to those previously reported with Giemsa R banding. In addition, sequential Q banding and R banding were carried out in the same cells. No significant differences in resolution of bands were observed when these two techniques were compared.