烟草叶片组织中肽酶活性与衰老的关系。
Activity of peptidase in tobacco-leaf tissue in relation to senescence.
作者信息
Anderson J W, Rowan K S
出版信息
Biochem J. 1965 Dec;97(3):741-6. doi: 10.1042/bj0970741.
Abstract
- Peptidase extracted with buffer at pH5.2 from small samples of tobacco-leaf tissue was assayed by measuring the alpha-amino nitrogen liberated from gelatin at 40 degrees in 1hr. The rate of hydrolysis was constant during the digestion. 2. The inclusion of sodium thioglycollate (60mm) in the extracting medium increased the activity of the peptidase. 3. As the major products of hydrolysis were peptides, the enzyme is an endopeptidase. 4. The decrease in protein observed in leaves with increasing physiological age and with time after harvest is correlated with increase in specific activity of peptidase in extracts from the leaves.
摘要
- 用pH5.2的缓冲液从小片烟草叶组织中提取的肽酶，通过测量在40℃下1小时内从明胶中释放出的α-氨基氮来进行测定。消化过程中水解速率恒定。2. 在提取介质中加入巯基乙酸钠（60mmol）可提高肽酶的活性。3. 由于水解的主要产物是肽，所以该酶是一种内肽酶。4. 随着叶片生理年龄的增加以及收获后时间的推移，叶片中观察到的蛋白质减少与叶片提取物中肽酶比活性的增加相关。
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