鸡肝核糖核酸对大鼠肝脏氨基酸掺入系统的影响。
The effect of chick-liver ribonucleic acid on amino acid-incorporation systems from rat liver.
作者信息
von der Decken A
出版信息
Biochem J. 1965 Dec;97(3):870-8. doi: 10.1042/bj0970870.
Abstract
- Rat-liver microsomes, ribonucleoprotein particles and a fraction mainly consisting of microsomal membranes were tested for their ability to incorporate amino acids into protein in the presence of ATP, GTP, phosphoenolpyruvate and pyruvate kinase. Addition of polyuridylic acid or of ribonucleic acid from rat-liver nuclei stimulated the incorporating activities. 2. These protein-synthesizing systems were found to be susceptible to ribonucleic acid from chick-liver nuclei as well. The biological activity of the ribonucleic acid from chick liver was measured by its capacity to stimulate amino acid incorporation. 3. In the presence of chick-liver ribonucleic acid, the ribonucleoprotein particles from rat liver showed an increased radioactivity in ribosomal units with a sedimentation constant higher than 70s. 4. This was investigated by sucrose-gradient centrifugation or by column chromatography on agarose suspensions.
摘要
- 在ATP、GTP、磷酸烯醇丙酮酸和丙酮酸激酶存在的情况下，对大鼠肝脏微粒体、核糖核蛋白颗粒以及主要由微粒体膜组成的部分进行了测试，以检测它们将氨基酸掺入蛋白质的能力。添加多聚尿苷酸或大鼠肝脏细胞核的核糖核酸可刺激掺入活性。2. 还发现这些蛋白质合成系统对鸡肝脏细胞核的核糖核酸也敏感。通过其刺激氨基酸掺入的能力来测量鸡肝脏核糖核酸的生物活性。3. 在鸡肝脏核糖核酸存在的情况下，大鼠肝脏的核糖核蛋白颗粒在沉降常数高于70s的核糖体单位中显示出放射性增加。4. 通过蔗糖梯度离心或在琼脂糖悬浮液上进行柱色谱法对此进行了研究。
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