尿激肽释放酶的直接测定法。
A direct assay for urinary kallikrein.
作者信息
Oza N B
出版信息
Biochem J. 1977 Oct 1;167(1):305-7. doi: 10.1042/bj1670305.
The first protein-binding assay is described for the direct determination of kallikrein in rat urine. Isolation of urinary kallikrein and preparation of its specific antibody have been previously described. Outlined here are the methods for 3H-labelling of kallikrein, for isolation of 3H-labelled immunoreactive enzyme, and for separation of free and antibody-bound kallikrein with the aid of double antibody. Assay and equilibrium conditions were characterized and a protocol is presented for the measurement of kallikrein concentration. The direct assay is sensitive, accurate and it correlates well (r = 0.81) with a functional assay. The assay may hold promise to determine catalytically active and inactive enzyme in urine, tissues and biological fluids.
首次描述了用于直接测定大鼠尿液中激肽释放酶的蛋白质结合测定法。尿激肽释放酶的分离及其特异性抗体的制备先前已有报道。这里概述了激肽释放酶的3H标记方法、3H标记免疫反应性酶的分离方法以及借助双抗体分离游离和抗体结合的激肽释放酶的方法。对测定和平衡条件进行了表征，并给出了测量激肽释放酶浓度的方案。该直接测定法灵敏、准确，与功能测定法相关性良好（r = 0.81）。该测定法有望用于测定尿液、组织和生物体液中具有催化活性和无活性的酶。
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