Mantovani A, Polentarutti N, Alessandri G, Vecchi A, Giuliani F, Spreafico F
Br J Cancer. 1977 Oct;36(4):453-60. doi: 10.1038/bjc.1977.214.
The effector arm of antibody-dependent cellular cytotoxicity (ADCC) was evaluated using 51Cr-labelled chicken erythrocytes as targets in BALB/c mice transplanted with the Moloney sarcoma virus-induced tumours T-MSV and MS2, and in C57BL/6 mice transplanted with the chemically induced FS6 sarcoma, Lewis lung carcinoma and B16 melanoma. Tumour-bearing animals showed higher levels of ADCC than normal mice, a stimulation confirmed in MS2-bearing mice, using SL2 lymphoma cells as targets in a cytostasis assay. ADCC effector-cell capacity was higher in animals transplanted with the immunogenic, spontaneously regressing T-MSV than in mice bearing the poorly immunogenic metastasizing MS2 sarcoma. The increased ADCC activity detectable in the spleen of tumour-bearing hosts was not abolished by removal of phagocytic-adherent cells.
使用51Cr标记的鸡红细胞作为靶标，在移植了莫洛尼肉瘤病毒诱导的肿瘤T-MSV和MS2的BALB/c小鼠，以及移植了化学诱导的FS6肉瘤、刘易斯肺癌和B16黑色素瘤的C57BL/6小鼠中，评估了抗体依赖性细胞毒性（ADCC）的效应臂。荷瘤动物的ADCC水平高于正常小鼠，在以SL2淋巴瘤细胞为靶标的细胞生长抑制试验中，对荷MS2小鼠的刺激得到了证实。移植免疫原性自发消退的T-MSV的动物的ADCC效应细胞能力高于荷低免疫原性转移性MS2肉瘤的小鼠。荷瘤宿主脾脏中可检测到的ADCC活性增加，不会因去除吞噬粘附细胞而消除。
