微管蛋白制剂的平衡成分。

Equilibrium components of tubulin preparations.

作者信息

Gethner J S, Flynn G W, Berne B J, Gaskin F

出版信息

Biochemistry. 1977 Dec 27;16(26):5781-5. doi: 10.1021/bi00645a021.

Abstract

Laser light scattering techniques are used to examine the existence of a tubulin-ring or tubulin-aggregate equilibrium. Three principal components in addition to tubulin dimer have been observed. One large component having D20,w = 0.65 +/- 0.25 X 10(-7) cm2/s is in equilibrium with tubulin (dimer) at 4 degrees C. A second large component having D20,w = 0.74 +/- 0.05 X 10(-7) cm2/s is metastable at 4 degrees C and is formed when tubulin solutions are warmed to 36 degrees C and cooled back to 4 degrees C. The properties of the component reported in the previous paper (Gethner, J.S., Flynn, G.W., Berne, B.J., and Gaskin, F. (1977), Biochemistry 16 (preceding paper in this issue)) to sediment at 10 S to 20 S are shown to be consistent with a small, metastable aggregate of tubulin.

摘要

激光光散射技术用于检测微管蛋白环或微管蛋白聚集体平衡的存在。除了微管蛋白二聚体之外，还观察到三个主要成分。一个大成分，其D20,w = 0.65 +/- 0.25×10(-7) cm2/s，在4℃下与微管蛋白（二聚体）处于平衡状态。另一个大成分，其D20,w = 0.74 +/- 0.05×10(-7) cm2/s，在4℃下是亚稳态的，当微管蛋白溶液加热到36℃然后冷却回4℃时形成。上一篇论文（Gethner, J.S., Flynn, G.W., Berne, B.J., and Gaskin, F. (1977), Biochemistry 16（本期之前的论文））报道的在10 S至20 S沉降的成分的性质表明与微管蛋白的一个小的亚稳态聚集体一致。