Akhrem A A, Abeliovich M L, Metelitsa D I
Biokhimiia. 1977 Nov;42(11):1978-82.
The kinetics of anisole oxidation by microsomes and tertiary butylhydroperoxide have been studied in phosphate buffer (pH 7,4) at 37 degrees C. About 20% of the substrate are shown to undergo demetylation and about 80% were hydroxylated in the p-position. The kinetics of the oxidative o-demethylation of anisole and p-chloroanisole by cumene hydroperoxide and hepatic microsomes has been studied within the temperature range of 20--37 degrees C. The catalytic rate constants for o-demethylation of anisole and chloroanisole have been determined in a general form to equal 2,04.10(7) exp (--11400/RT) and 5,5.10(7) exp (--11800/RT) sec-1, respectively. The reactions of o-demethylation and hydroperoxide have been proved to be independent. The data obtained are indicative of the electrophilic nature of the hydroxylating agent in the system "microsomes-hydroperoxide".
在37℃的磷酸盐缓冲液（pH 7.4）中，研究了微粒体和叔丁基过氧化氢对苯甲醚的氧化动力学。结果表明，约20%的底物发生脱甲基反应，约80%在对位发生羟基化反应。在20-37℃的温度范围内，研究了异丙苯过氧化氢和肝微粒体对苯甲醚和对氯苯甲醚的邻位氧化脱甲基动力学。已确定苯甲醚和氯苯甲醚邻位脱甲基的催化速率常数一般形式分别为2.04×10⁷ exp（-11400/RT）和5.5×10⁷ exp（-11800/RT）秒⁻¹。邻位脱甲基反应和过氧化氢反应已被证明是相互独立的。所得数据表明“微粒体-过氧化氢”体系中羟化剂的亲电性质。
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