Metelitza D I, Akhrem A A, Erjomin A N, Kissel M A, Usanov S A
Acta Biol Med Ger. 1979;38(2-3):511-8.
Cytochrome P-450 destruction kinetics by cumene hydroperoxide (CHP) has been studied at 25 degrees C in phosphate buffer, pH 7.25-7.50, in various systems: intact and induced rat or rabbit microsomes, highly purified LM2- and LM2- and LM4-forms of cytochrome P-450 from rabbit liver microsomes. The destruction kinetics is characterized by three phases in all systems. The CHP-influenced cytochrome P-450 destruction is a radical chain process with linear termination of the chains. The acidic phospholipids, phosphatidylserine and phosphatidylinositol and total microsomal phospholipids containing the acidic lipid components activate cytochrome P-450 in the hydroxylation of aniline and naphthalene by CHP. Phosphatidylcholine and sphingomyelin have no effect upon the cytochrome P-450 activity in the type I and II substrates oxidation by CHP. The phase transitions of the microsomal phospholipids influence the interaction of cytochrome P-450 with its reductase, altering the activation energy of type I substrates oxidation. The type II substrate oxidation is not affected by phase transitions in the full microsomal hydroxylating system.
在25摄氏度下，于pH值为7.25 - 7.50的磷酸盐缓冲液中，在各种体系中研究了氢过氧化异丙苯（CHP）对细胞色素P - 450的破坏动力学：完整的及诱导的大鼠或兔微粒体、来自兔肝微粒体的高度纯化的细胞色素P - 450的LM2 - 和LM4 - 形式。在所有体系中，破坏动力学均以三个阶段为特征。CHP影响的细胞色素P - 450破坏是一个自由基链式过程，链以线性方式终止。酸性磷脂、磷脂酰丝氨酸和磷脂酰肌醇以及含有酸性脂质成分的总微粒体磷脂在CHP对苯胺和萘的羟基化反应中激活细胞色素P - 450。磷脂酰胆碱和鞘磷脂对CHP在I型和II型底物氧化中细胞色素P - 450的活性没有影响。微粒体磷脂的相变影响细胞色素P - 450与其还原酶的相互作用，改变I型底物氧化的活化能。在完整的微粒体羟基化体系中，II型底物氧化不受相变的影响。
