Study of carboxyl groups on the human blood platelet membrane.

The authors have studied the whole carboxyl groups of the platelet membrane using liquid phase electrophoresis and an initial step of activation of the acid group by a water soluble carbodiimide (ethyl-l-diméthyl-aminopropyl-3-carbodiimide), followed by the action of a nucleophilic molecule. The calculation based on the theory of Gouy-Chapman have shown the existence of 24, 7.10(5) groups per cell. That is a number about 2.5 fold superior to the carboxyl groups of sialic acid as determined by the action of neuraminidase.