Dankwarth L, Messer W
J Immunol Methods. 1976 Mar;10(2-3):127-32. doi: 10.1016/0022-1759(76)90163-0.
Spleen cells from mice immunized with E. coli beta-galactosidase were incubated with the enzyme and with 5-bromo-4-chloroindolyl-beta-galactosidase as a histochemical substrate. Some cells are stainable in this reaction, however, not all of them carry specific receptors for beta-galactosidases. Some spleen cells can take up dye and thereby become unspecifically stained. This system is therefore not suitable for the detection of antigen binding to cells.
用大肠杆菌β-半乳糖苷酶免疫的小鼠脾脏细胞与该酶以及作为组织化学底物的5-溴-4-氯吲哚基-β-半乳糖苷一起孵育。在这个反应中，一些细胞是可染色的，然而，并非所有细胞都携带β-半乳糖苷酶的特异性受体。一些脾脏细胞可以摄取染料，从而被非特异性染色。因此，该系统不适用于检测与细胞结合的抗原。
