Patterson R, Chakrin L W, Suszko I M, Mengel J, Wardell J R
J Lab Clin Med. 1976 Jun;87(6):1016-24.
Peripheral blood leukocytes (PBL) and free respiratory cells (RC) of the mast cell-basophil type were obtained from normal rhesus monkeys or rhesus monkeys with defined immediate-type hypersensitivity to ascaris antigen (AA). PBL or RC from the latter were exposed to AA and the former were exposed to anti-IgE. Histamine (H) release and release of a slow reacting substance of anaphylaxis (SRS-A) occurred following exposure of the appropriate cells to AA or anti-IgE. The release of both H and SRS-A from PBL was potentiated by D2O. D2O did not potentiate release of SRS-A or H from RC due to anti-IgE to the same extent. Although potentiation of H release from RC due to AA was observed in the presence of D2O in some individual animals, this could not be demonstrated by statistical analysis of group results. We could not demonstrate potentiation of SRS-A release from RC due to anti-IgE or AA. The results suggest the possibility that there may be differences in antigen reactive cells of the respiratory tree and peripheral blood and biochemical studies may be of use in demonstrating these differences.
从正常恒河猴或对蛔虫抗原（AA）具有明确速发型超敏反应的恒河猴中获取外周血白细胞（PBL）和肥大细胞 - 嗜碱性粒细胞类型的游离呼吸细胞（RC）。将后者的PBL或RC暴露于AA，前者暴露于抗IgE。将相应细胞暴露于AA或抗IgE后，发生组胺（H）释放和过敏反应慢反应物质（SRS - A）释放。D2O增强了PBL中H和SRS - A的释放。D2O对RC因抗IgE导致的SRS - A或H释放的增强程度相同。尽管在某些个体动物中，在D2O存在的情况下观察到AA导致RC中H释放增强，但通过对组结果的统计分析无法证实这一点。我们无法证实抗IgE或AA导致RC中SRS - A释放增强。结果表明，呼吸树和外周血的抗原反应性细胞可能存在差异，生化研究可能有助于证明这些差异。
