Schleyer M, Voigt K H
Hoppe Seylers Z Physiol Chem. 1977 Dec;358(12):1557-64. doi: 10.1515/bchm2.1977.358.2.1557.
A method for preparation of highly purified human somatotropin on large-scale basis is described. Starting from deep-frozen pituitary gland, less time is needed to obtain highly purified hormone than with other published methods for preparation of human somatotropin. The hormone obtained in this fasion is chromatographically and electrophoretically homogeneous; it shows high biological and radioimmunological growth hormone activity and is free of other pituitary hormone activities. The effects of various experimental conditions upon aggregation of somatotropin are critically evaluated.
本文描述了一种大规模制备高纯度人生长激素的方法。从深度冷冻的垂体开始，与其他已发表的人生长激素制备方法相比，获得高纯度激素所需的时间更短。以这种方式获得的激素在色谱和电泳上是均一的；它显示出高生物学和放射免疫生长激素活性，且不含其他垂体激素活性。对各种实验条件对生长激素聚集的影响进行了严格评估。
