4-二甲基氨基-3'-甲基偶氮苯对大鼠肝脏多核糖体制剂掺入14-C标记氨基酸的影响。

The effect of 4-dimethylamino-3'-methylazobenzene on 14-C-labelled amino acid incorporation by rat-liver polysome preparations.

作者信息

Hawtrey A O, Nourse L D

出版信息

Biochem J. 1966 Mar;98(3):682-8. doi: 10.1042/bj0980682.

DOI:10.1042/bj0980682

PMID:5911517

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1264907/

Abstract

The incorporation of (14)C-labelled amino acids into polysomal protein was studied in a system consisting of polysomes and pH5 enzyme obtained 4 and 40hr. after a single intraperitoneal injection of 4-dimethylamino-3'-methylazobenzene. Labelling of the polysome fraction of preparations of both the 4hr.-treated and 40hr.-treated rats was considerably higher than in the normal control. 2. In further experiments on protein synthesis by polysomes from azo-dye-treated rats, the effect of replacing pH5 enzyme with cell sap was studied. Incorporation of (14)C-labelled amino acids into polysomal protein was similar to that of the control. 3. Aggregate size of polysomes obtained from rats injected previously with 4-dimethylamino-3'-methylazobenzene was studied by sucrose-gradient centrifugation. Polysomes prepared at 4hr. after azo-dye administration contained a considerable amount of large aggregates (approx. 700s), whereas at 40hr. after administration of the azo-dye the amount of large aggregates was less than in the control. 4. Determination of the ultraviolet spectra of polysome preparations from both normal and azo-dye-treated rats revealed no difference between the preparations. On the other hand, the ultraviolet spectra of cell-sap fractions from the different preparations showed that there is a definite shift in the absorption maximum from 272mmu (normal) to 267mmu, 40hr. after treatment, with an intermediate value of 270mmu for the preparation from 4hr.-treated rats. The absorption minimum changes from 250mmu (normal) to 245mmu for the preparation from 40hr.-treated rats.

摘要

在一个由多核糖体和pH5酶组成的系统中，研究了（14）C标记的氨基酸掺入多核糖体蛋白的情况。该系统中的多核糖体和pH5酶是在单次腹腔注射4-二甲基氨基-3'-甲基偶氮苯后4小时和40小时获得的。4小时处理组和40小时处理组大鼠制剂的多核糖体部分的标记率明显高于正常对照组。2. 在关于偶氮染料处理大鼠的多核糖体进行蛋白质合成的进一步实验中，研究了用细胞液替代pH5酶的效果。（14）C标记的氨基酸掺入多核糖体蛋白的情况与对照组相似。3. 通过蔗糖梯度离心研究了先前注射4-二甲基氨基-3'-甲基偶氮苯的大鼠获得的多核糖体的聚集体大小。在偶氮染料给药后4小时制备的多核糖体含有大量大聚集体（约700s），而在给药后40小时，大聚集体的数量少于对照组。4. 对正常和偶氮染料处理大鼠的多核糖体制剂的紫外光谱测定表明，这些制剂之间没有差异。另一方面，不同制剂的细胞液部分的紫外光谱表明，处理后40小时，吸收最大值从272nm（正常）有一个明确的位移至267nm，4小时处理组大鼠制剂的中间值为270nm。40小时处理组大鼠制剂的吸收最小值从250nm（正常）变为245nm。