一种用于测定血液中各种成分里核黄素的光分解荧光法。
A photodecomposition fluorimetric method for determination of riboflavin in the various constitutents of blood.
作者信息
Clarke H C
出版信息
Int J Vitam Nutr Res. 1977;47(4):356-60.
PMID:591206
Abstract
The photodecomposition fluorimetric method has been modified for determination of small quantities of whole blood, erythrocytes, leucocytes, platelets and plasma. The method is accurate in each constituent and contains a test of specificity in each determination. Automation of the method is described. In 13 normal subjects riboflavin in whole blood was range 14.5-24.2, mean 17.7 +/- 2.7 microgram %, in corresponding erythrocytes was range 21.0-44.0, mean 33.7 +/- 6.6 microgram%.
摘要
已对光分解荧光法进行了改进，用于测定少量全血、红细胞、白细胞、血小板和血浆。该方法对每种成分的测定都很准确，且每次测定都包含特异性检测。文中描述了该方法的自动化过程。在13名正常受试者中，全血中核黄素含量范围为14.5 - 24.2，平均为17.7±2.7微克%，相应红细胞中核黄素含量范围为21.0 - 44.0，平均为33.7±6.6微克%。
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