Gel filtration analysis of vanadium in Ascidia nigra blood cell lysate.
作者信息
Gilbert K, Kustin K, McLeod G C
出版信息
J Cell Physiol. 1977 Nov;93(2):309-11. doi: 10.1002/jcp.1040930217.
PMID:591565
Abstract
Fractions from a Sephadex gel filtration of homogenized Ascidia nigra blood cell lysate were analyzed for vanadium by atomic absorption spectroscopy. The results were unaffected by temperature from 4-21 degrees C, and by ionic strength in the range 0.09-1.0 M (NaCl). Appreciable loss of vanadium in the supernatant was experienced above pH 2.2. Experiments at pH 2.1 under anaerobic conditions show that the green chromogen and the vanadium-containing bands elute separately. Under these experimental conditions, the vanadium-containing species is of relatively low (congruent to 1,000) molecular weight.
摘要
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