鲎单感光细胞中视紫红质分子数量的电生理测量。
Electrophysiological measurement of the number of rhodopsin molecules in single Limulus photoreceptors.
作者信息
Lisman J E, Bering H
出版信息
J Gen Physiol. 1977 Nov;70(5):621-33. doi: 10.1085/jgp.70.5.621.
Abstract
Two partly independent electrophysiological methods are described for measuring the number of rhodopsin molecules (R) in single ventral photoreceptors. Method 1 is based on measurements of the relative intensity required to elicit a quantal response and the relative intensity required to half-saturate the early receptor potential (ERP). Method 2 is based on measurements of the absolute intensity required to elicit a quantal response. Both methods give values of R approximately equal to 10(9). From these and other measurements, estimates are derived for the surface density of rhodopsin (8,000/micrometer2), the charge movement during the ERP per isomerized rhodopsin (20 X 10(-21) C), and the half-time for thermal isomerization of rhodopsin (36yr).
摘要
描述了两种部分独立的电生理方法，用于测量单个腹侧光感受器中视紫红质分子（R）的数量。方法1基于对引发量子反应所需的相对强度以及使早期感受器电位（ERP）半饱和所需的相对强度的测量。方法2基于对引发量子反应所需的绝对强度的测量。两种方法得出的R值大约等于10⁹。根据这些以及其他测量结果，得出了视紫红质的表面密度（8000/微米²）、每个异构化视紫红质在ERP期间的电荷移动（20×10⁻²¹库仑）以及视紫红质热异构化的半衰期（36年）的估计值。
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