Koziorowska J, Wlodarski K
J Exp Med. 1966 Aug 1;124(2):199-208. doi: 10.1084/jem.124.2.199.
A morphologic study has been made on KB cells infected with various doses of vaccinia as to DNA synthesis and mitosis. Determination of mitotic indices revealed that the mitotic cell pool depended on the proportion of infected cells and the time after infection. By cytologic examination neither mitotic lesions were found nor an accumulation of mitotic cells at any one stage of mitosis was demonstrated. Radioautographs of infected cultures have shown that the frequency of cells labeled over nuclei was significantly increased as compared with control cultures. Following the greatest dose of virus (multiplicity of 20 PFU/cell) the ratio of cells synthesizing DNA to mitotic cells increased from 45:5 at 5 hr to 50:0 at 50 hr. Concommittant with the appearance of this disparity between the DNA-synthesizing cell pool and mitotic cell pool the nuclei of cells became "lightly" labeled. Following the lowest dose of virus (multiplicity of 0.0002 PFU/cell) the increase of the fraction of mitotic cells was proportional to the increase of the fraction of cells which were labeled over nuclei.
针对感染不同剂量痘苗的KB细胞的DNA合成和有丝分裂进行了形态学研究。有丝分裂指数的测定表明，有丝分裂细胞库取决于感染细胞的比例和感染后的时间。通过细胞学检查，未发现有丝分裂损伤，也未证明在有丝分裂的任何一个阶段有丝分裂细胞的积累。感染培养物的放射自显影片显示，与对照培养物相比，细胞核被标记的细胞频率显著增加。在最大剂量的病毒（感染复数为20个蚀斑形成单位/细胞）作用后，合成DNA的细胞与有丝分裂细胞的比例从5小时时的45:5增加到50小时时的50:0。随着DNA合成细胞库和有丝分裂细胞库之间这种差异的出现，细胞的细胞核被“轻度”标记。在最低剂量的病毒（感染复数为0.0002个蚀斑形成单位/细胞）作用后，有丝分裂细胞分数的增加与细胞核被标记的细胞分数的增加成比例。
