培养的哺乳动物细胞中辐射刺激的DNA合成
Radiation-stimulated DNA synthesis in cultured mammalian cells.
作者信息
Rasmussen R E, Painter R B
出版信息
J Cell Biol. 1966 Apr;29(1):11-9. doi: 10.1083/jcb.29.1.11.
Abstract
A type of DNA synthesis in mammalian cells that is stimulated by ultraviolet light has been studied by means of radioautography and density gradient centrifugation. The characteristics of this synthesis are: (a) it is not semiconservative; (b) it is enhanced by the presence of 5-bromodeoxyuridine in the DNA molecule; (c) the degree of stimulation is dose dependent; (d) there is less variability in the rate of incorporation of H(3)-thymidine during this synthesis than during normal DNA synthesis; (e) it occurs in cells that are not in the normal DNA synthesis phase (G(1) and G(2) cells). This kind of synthesis has been found in cultured cell lines from five different species; however, in some strains, the presence of bromouracil in the DNA is required before it can be demonstrated by radioautography.
摘要
通过放射自显影术和密度梯度离心法，对哺乳动物细胞中一种受紫外线刺激的DNA合成类型进行了研究。这种合成的特点是：（a）它不是半保留性的；（b）DNA分子中5-溴脱氧尿苷的存在会增强这种合成；（c）刺激程度呈剂量依赖性；（d）与正常DNA合成相比，这种合成过程中H(3)-胸苷掺入速率的变异性较小；（e）它发生在处于非正常DNA合成阶段的细胞（G(1)和G(2)期细胞）中。在来自五个不同物种的培养细胞系中都发现了这种合成；然而，在某些菌株中，在通过放射自显影术证明之前，DNA中需要存在溴尿嘧啶。
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