Lavergne G M, James H F, Martineau C, Diena B B, Lior H
Lab Anim Sci. 1977 Oct;27(5 Pt 2):806-16.
Studies were designed to develop an animal model which mimics the asymptomatic carrier state of typhoid fever, Salmonella typhi infection, in man. The gallbladder of the guinea pig [Can (HA)] was surgically cannulated to provide a route for infection and bile sampling. Organisms (2 X 10(8) S typh:) were introduced by way of the cannula and samples of bile removed and analyzed periodically. Salmonella typhi was recovered from the bile and feces of infected, yet apparently normal, guinea pigs for periods of up to 5 months thus mimicking the human symptomeles carrier. Some guinea pigs were treated with chloramphenicol after infection. Antibiotic therapy with chloramphenicol did not eliminate the carrier state. Microabscesses of the liver were induced by S typhi both in untreated and treated animals (cultures were positive from these abscesses). When blood antibiotic levels dropped, the organism was found in the bile fluid and was again excreted. Vaccinated guinea pigs developed elevated levels of 0 and H serum antibody, but were not protected against infection of the gallbladder with S typhi (2 X 10(8) organisms). Similarly, vaccination failed to eliminate the carrier state.
研究旨在建立一种动物模型，该模型可模拟人类伤寒热（伤寒杆菌感染）的无症状带菌状态。对豚鼠[Can(HA)]的胆囊进行手术插管，以提供感染途径和胆汁采样途径。通过插管引入细菌（2×10⁸伤寒杆菌），并定期采集胆汁样本进行分析。在长达5个月的时间里，从感染但表面正常的豚鼠的胆汁和粪便中分离出伤寒杆菌，从而模拟人类无症状带菌者。一些豚鼠在感染后接受氯霉素治疗。氯霉素抗生素治疗并未消除带菌状态。在未治疗和治疗的动物中，伤寒杆菌均诱发了肝脏微脓肿（这些脓肿培养呈阳性）。当血液抗生素水平下降时，在胆汁液中发现该细菌，并再次排出。接种疫苗的豚鼠血清中0和H抗体水平升高，但未受到针对伤寒杆菌（2×10⁸个细菌）胆囊感染的保护。同样，接种疫苗未能消除带菌状态。
