Webster D A, Hackett D P
Plant Physiol. 1966 Apr;41(4):599-605. doi: 10.1104/pp.41.4.599.
Whole cell difference spectra of the blue-green algae, Saprospira grandis, Leucothrix mucor, and Vitreoscilla sp. have one, or at the most 2, broad alpha-bands near 560 mmu. At -190 degrees these bands split to give 4 peaks in the alpha-region for b and c-type cytochromes, but no alpha-band for a-type cytochromes is visible. The NADH oxidase activity of these organisms was shown to be associated with particulate fractions of cell homogenates. The response of this activity to inhibitors differed from the responses of the NADH oxidase activities of particulate preparations from the green algae and higher plants to the same inhibitors, but is more typical of certain bacteria. No cytochrome oxidase activity was present in these preparations. The respiration of Saprospira and Vitreoscilla can be light-reversibly inhibited by CO, and all 3 organisms have a CO-binding pigment whose CO complex absorbs near 570, 535, and 417 mmu. The action spectrum for the light reversal of CO-inhibited Vitreoscilla respiration shows maxima at 568, 534, and 416 mmu. The results suggest that the terminal oxidase in these blue-greens is an o-type cytochrome.
蓝绿藻、大腐螺旋菌、粘液亮发菌和透明颤菌的全细胞差异光谱在560毫微米附近有一条或最多两条宽的α带。在-190℃时，这些带分裂，在α区域给出b型和c型细胞色素的4个峰，但未观察到a型细胞色素的α带。已表明这些生物体的NADH氧化酶活性与细胞匀浆的颗粒部分有关。这种活性对抑制剂的反应不同于绿藻和高等植物颗粒制剂的NADH氧化酶活性对相同抑制剂的反应，但更具某些细菌的典型特征。这些制剂中不存在细胞色素氧化酶活性。腐螺旋菌和透明颤菌的呼吸作用可被CO光可逆抑制，并且所有3种生物体都有一种与CO结合的色素，其CO复合物在570、535和417毫微米附近吸收。CO抑制的透明颤菌呼吸作用的光逆转作用光谱在568、534和416毫微米处出现最大值。结果表明这些蓝绿藻中的末端氧化酶是一种o型细胞色素。
