胚胎鸡晶状体细胞中焦磷酸酶活性从mRNA 5'末端帽结构中切割pm7G。
Cleavage of pm7G from mRNA 5' terminal cap structures by pyrophosphatase activity in embryonic chick lens cells.
作者信息
Lavers G C
出版信息
Mol Biol Rep. 1977 Dec;3(6):413-20. doi: 10.1007/BF00808382.
PMID:593271
Abstract
The presence of pyrophosphatase activity in embryonic lens cells which cleaves pm7G and ppGm from m7G(5')pppGm was demonstrated. It was also found that m7G(5')pppG, but not G(5')pppG, was hydrolyzed, and conversion of m7GpppG to m7G*pppG, in which the 5-membered ring of the m7G moiety is open, abolished its hydrolysis. For the caps hydrolyzed, pm7G was released only in the presence of lens cellular fraction; pm7G inhibited cap hydrolysis.
摘要
已证实胚胎晶状体细胞中存在焦磷酸酶活性，该酶可从m7G(5')pppGm中切割出pm7G和ppGm。还发现m7G(5')pppG可被水解，而G(5')pppG则不能，并且m7GpppG转化为m7G*pppG（其中m7G部分的五元环是开放的）后，其水解作用被消除。对于被水解的帽结构，仅在存在晶状体细胞组分的情况下才会释放出pm7G；pm7G可抑制帽结构的水解。
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