Frist S, Wenz B
Transfusion. 1976 May-Jun;16(3):261-6. doi: 10.1046/j.1537-2995.1976.16376225501.x.
Renal allograft rejection, following development of a positive indirect antiglobulin reaction, occurred in a multiply transfused recipient. Panel techniques confirmed both anti-Bga and anti-Kell antibodies. Recognition of the concordant antigenic specificity of the Bga and HL-A7 antigens led to an investigation of the potential role of this antibody in the rejection phenomenon. In the absence of serological cytotoxicity, a modified elution technique was used to directly obtain immunoglobulin from the rejected allograft. The eluate obtained displayed specificity for the Bga red blood cell antigen. The described technique affords an additional approach to the documentation of immunologically mediated graft rejection and obviates the limitations imposed by the absence of serological cytotoxicity. Emphasis is placed on the need for recognition of the relationship between red blood cell and HL-A antigens.
在一位多次输血的受者中，出现了阳性间接抗球蛋白反应后发生了肾移植排斥反应。谱细胞技术证实了抗Bga和抗Kell抗体。认识到Bga和HL - A7抗原的一致抗原特异性后，对该抗体在排斥现象中的潜在作用进行了研究。在没有血清学细胞毒性的情况下，采用改良洗脱技术直接从被排斥的移植肾中获取免疫球蛋白。所获得的洗脱液显示出对Bga红细胞抗原的特异性。所描述的技术为免疫介导的移植排斥反应的记录提供了另一种方法，并且避免了因缺乏血清学细胞毒性而带来的限制。重点强调了认识红细胞与HL - A抗原之间关系的必要性。
